Phenotypic and genotypic characterization of carbapenem-resistant Gram-negative organisms, Beni-Suef, Egypt
نویسندگان
چکیده
Abstract Background Carbapenem-resistant Gram-negative organisms (CRGNO) are a growing threat. We aimed in our study to determine the genotype of carbapenemases at Beni-Suef University Hospital by using newly introduced lateral flow assays comparison with molecular techniques and test effectiveness ceftazidime/avibactam against them. Methods Screening for carbapenemase production was done mSuperCARBA (CHROMagar™ company). Genotypic characterization 3 different kits assays: NG-Test CARBA5 assay (NG Biotech, Guipry, France), RESIST-3 O.K.N. (Coris BioConcept, Belgium) K.N.I.V.O Detection K-Set (Beijing Gold Mountain river Tech Development Co, China), whereas genotypic blaVIM blaIMP, blaKPC, blaOXA-48, blaNDM PCR. Results The high prevalence CRGNO (29%) dominated Klebsiella pneumonia (83.3%) harboring OXA-48 (92%). Lateral immunoassays showed sensitivity specificity each type Conclusion coexistence multiple genes same isolate increased resistance new therapeutic options, e.g., CZA/AVI. Proper implementation isolation measures health care facilities can render spread CRGNO.
منابع مشابه
Phenotypic and Genotypic Detection of Metallo-Beta-Lactamases in Carbapenem Resistant Acinetobacter baumannii
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phenotypic and genotypic detection of metallo-beta-lactamases among imipenem resistant gram negative isolates
background : imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams....
متن کاملphenotypic and genotypic detection of metallo-beta-lactamases among imipenem-resistant gram negative isolates
background : imipenem-resistant gram negative bacteria, resulting from metallo-beta-lactamase (mbls)-producing strains have been reported to be among the important causes of nosocomial infections and of serious therapeutic problem worldwide. because of their broad range, potent carbapenemase activity and resistance to inhibitors, these enzymes can confer resistance to almost all beta-lactams....
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ژورنال
عنوان ژورنال: Beni-Suef University Journal of Basic and Applied Sciences
سال: 2023
ISSN: ['2314-8543', '2314-8535']
DOI: https://doi.org/10.1186/s43088-023-00400-w